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KMID : 1144820190250040302
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대한의생명과학회지
2019 Volume.25 No. 4 p.302 ~ p.312
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Anti-platelet Effect of Black Tea Extract via Inhibition of TXA2 in Rat
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Ro Ju-Ye
Cho Hyun-Jeong
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Abstract
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The aim of this work was to investigate the effect of black tea extract (BTE) on collagen-induced platelet aggregation. In this study, BTE (10~500 μg/mL) was shown to inhibit platelet aggregation via thromboxane A2 (TXA2) downregulation by blocking cyclooxygenase-1 (COX-1) activity. Also, BTE decreased intracellular Ca2+ mobilization ([Ca2+]i). Additionally, BTE enhanced the levels of both cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which are aggregation-inhibiting molecules. BTE inhibited the phosphorylation of phospholipase C (PLC) γ2 and syk activated by collagen. BTE regulated platelet aggregation via cAMP-dependent phosphorylation of vasodilator-stimulated phosphoprotein (VASP) Ser157. The anti-platelet effects of BTE in high fat diet (HFD)-induced obese rats were evaluated. After eight weeks of BTE treatment (300 and 600 mg/kg), the platelet aggregation rate in the treated groups was significantly less than that in the HFD-fed control group. Also, BTE exhibited a hepatoprotective effect and did not exert hepatotoxicity. Therefore, these data suggest that BTE has anti-platelet effects on collagen-stimulated platelet aggregation and may have therapeutic potential for the prevention of platelet-mediated thrombotic diseases.
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KEYWORD
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Black tea extract, Cyclic nucleotides, Intracellular Ca2+, Platelet aggregation, Thromboxane A2
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